Meet Our Protein Characterization Problem Solver
Simple Western (JESS™)
Gel-free, blot-free, hands-free capillary-based immunoassay platforms
Your Complete Protein Analysis Solution
Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Just load your samples and reagents into the microplate and Jess separates your proteins by size, and precisely manages antibody additions, incubations, washes and even the detection steps. Come back to fully analyzed results in 3 hours. Go further with high sensitivity multiplexing—her best-in-class fluorescent detection channels and RePlexing capabilities get you all the information you need on your samples in one run.
What Jess can do for you?
- Separate and analyze 2-440 kDa proteins by size or charge
- Multiplex across 3 channels
- Picogram-level sensitivity with just 3 μl, 5 μl, or 12 μl of sample for charge-based separation
- RePlex: Run two immunoassays within the same capillary to get more data per sample
- Total Protein Detection: Normalize protein expression data with confidence
Applications
Specifications
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| Description | Total Protein | Immunoassay (Size) | Immunoassay (Charge) |
|---|---|---|---|
| Sample required | 0.3-1.2 µg lysate | 0.6-1.2 µg lysate | 0.6-1.2 µg |
| Dynamic range | 2-3 logs | up to 4 logs | 3 logs |
| Resolution | ±15-20% <20 kDa, ±10% >20 kDa | ±0.1 pI units | |
| Size or pI range | 2-40, 12-230, or 66-440 kDa | pI 3 to pI 10 | |
| Intra-assay CV | <15% | <20% | |
| Sensitivity | Mid pg with 5X Total Protein | Low pg | |
| Sizing CV | <10% | ||
| Inter-assay CV | <20% | ||
| Quantitation CV | <20% | ||
| Capillary | 5 cm, 100 µm, 400 nL | ||
Talk to Our Scientists
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