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Spatial Biology

RNA & DNA Target Detection in tissues and Single Cells

 

RNAscope™ Plus smRNA-RNA Assay

  • Unlock the potential to detection 1 small RNA along with up to 3 mRNA targets
  • Assess small RNA therapeutic delivery mechanism
  • Evaluate biodistribution and efficacy of therapy
  • Add visual dimension to heterogeneous tissue biology and analysis

RNAscope

  • ~20 ZZ pairs per target
  • Single RNA molecule detection
  • mRNA or IncRNA target (>300nt)
  • 1-plex or Duplex chromogenic: Automated and Manual
  • Up to 4-plex fluorescent: Automated and Manual
  • Up to 12-plex fluorescent: Manual
Compatible with RNA Protein Co-Detection Assays
 
 

Basescope

  • 1-3 ZZ pairs
  • Single RNA molecule detection
  • Splice variants, short/highly homologous sequences, and point mutations (50-300nt)
  • 1-plex chromogenic: Automated and Manual
  • Duplex chromogenic: Manual
Compatible with RNA Protein Co-Detection Assays
 
 

miRNAscope

  • Visualize smRNA and mRNA in single slide
  • RNA molecules at single cell resolution
  • ASOs, miRNAs, siRNAs, and other smaller RNA sequences (17-50nt)
  • 1-plex chromogenic: Automated and Manual
  • 1 smRNA and up to 3 mRNA: Fluorescent
Compatible with RNA Protein Co-Detection Assays
 
 

DNAscope

  • Leverage the ZZ design strategy
  • Utilize 2 orthogonal signal amplification systems to detect 2 DNA targets
  • Application focus on break-apart and copy number variation
  • Duplex chromogenic assay
 

Detection of anti-BCMA CAR-T cells, Inteferon-g expression by RNAscope multiplex fluorescence, and CD3+ T cells by immunofluorescence

Cell Therapy

CAR-T and TCR-T cell, as well as NK cell and macrophage therapies are being developed for treatment of solid tumor. RNAscope in situ hybridization is the only method that enables specific detection of these viral vector-based cell therapies in intact human tumor biopsies.


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Applications
Cell & Gene Therapy
Cancer
Viral
Neuroscience
RNA & Protein

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