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Spatial RNA & DNA Target Detection in tissues and Single Cells

 
 

RNAscope

  • ~20 ZZ pairs per target
  • Single molecule detection
  • mRNA or IncRNA target (>300nt)
  • 1-plex or Duplex chromogenic: Automated and Manual
  • Up to 4-plex fluorescence: Automated and Manual
  • Up to 12-plex fluorescence: Manual
Compatible with RNA Protein Co-Detection Assays
 
 

Basescope

  • 1-3 ZZ pairs
  • Single molecule detection
  • Splice variants, short/highly homologous sequences, and point mutations (50-300nt)
  • 1-plex chromogenic: Automated and Manual
  • Duplex chromogenic: Manual
Compatible with RNA Protein Co-Detection Assays
 
 

miRNAscope

  • New proble design strategy
  • RNA molecules at single cell resolution
  • ASOs, miRNAs, siRNAs, and other smaller RNA sequences (17-50nt)
  • 1-plex chromogenic: Automated and Manual
Compatible with RNA Protein Co-Detection Assays
 
 

DNAscope

  • Leverage the ZZ design strategy
  • Utilize 2 orthogonal signal amplification systems to detect 2 DNA targets
  • Application focus on break-apart and copy number variation
  • Duplex chromogenic assay
 

Detection of anti-BCMA CAR-T cells, Inteferon-g expression by RNAscope multiplex fluorescence, and CD3+ T cells by immunofluorescence

Cell Therapy

CAR-T and TCR-T cell, as well as NK cell and macrophage therapies are being developed for treatment of solid tumor. RNAscope in situ hybridization is the only method that enables specific detection of these viral vector-based cell therapies in intact human tumor biopsies.


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Applications
Cell & Gene Therapy
Cancer
Viral
Neuroscience
RNA & Protein

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